preservative (antifungal agent) INS 235

Natamycin E235

natural antimicrobial — Primarily NATURAL (produced by bacteria).

🇪🇺 EU: Approved
🇺🇸 USA: Approved
🇯🇵 Japan: Approved
🇦🇺 AU/NZ: Approved
🇨🇦 Canada: Approved
Scientific Name

Natamycin (polyene macrolide antibiotic)

CAS: 7681-93-8

Data verified: 2026-04-04

Factual Regulatory Reference

This database provides factual regulatory information compiled from official government sources. It does not constitute medical, nutritional, or safety advice. Regulatory status varies by country and is subject to change. Always refer to your local regulatory authority for the most current information.

? Did You Know?

Natamycin occurs naturally in Produced by soil bacterium Streptomyces natalensis. Many people consume it daily without realizing it's also a listed food additive.

Natamycin is produced through fermentation — the same biological process used to make bread, beer, and yogurt.

Natamycin isn't just a food additive — it's also used in medicine.

Detailed Regulatory Assessment

🇪🇺

European Union (EFSA)

approved Max: 1 mg/dm² (surface treatment) mg/kg

RESTRICTED to surface treatment of cheese and dried sausages ONLY; not for internal use

Official EFSA Link
🇺🇸

United States (FDA)

approved GRAS

Approved for surface treatment of cheese and dried sausages

🇯🇵

Japan (MHLW)

approved Cat: 既存添加物

Approved as existing food additive (natural origin)

Acceptable Daily Intake (ADI)

International Standard (JECFA)

0–0.3 mg/kg bw/day

mg/kg body weight per day

European Standard (EFSA)

0.3 mg/kg bw/day

Everyday Perspective

For a 60kg adult, this limit is roughly equivalent to consuming:

!
~60 of servings of surface-treated cheese (30g)
~0.3mg per serving
!
~36 of servings of dried sausage (50g)
~0.5mg per serving

Natural Occurrence

NATURAL antifungal produced by soil bacterium Streptomyces natalensis. Named after Natal, South Africa where the bacteria was discovered. Also called 'pimaricin' (from Pietermaritzburg, South Africa). Works ONLY on the surface - not meant to penetrate into food. The white coating you see on some cheese rinds is often natamycin. Extremely effective against yeasts and molds but has NO effect on bacteria. Does NOT promote antibiotic resistance because it's only used on surfaces and breaks down quickly. Used in medicine as antifungal for eye infections (not taken orally).

Produced by soil bacterium Streptomyces natalensis

Manufacturing

Method: natural fermentation

Produced by fermentation using Streptomyces natalensis bacteria, then purified and concentrated for use as a surface treatment antifungal.

Applications Beyond Food

Medical

Used as antifungal medication for eye infections (ophthalmic use)

Frequently Asked Questions

What is Natamycin (E235)?
Natamycin (E235) is a preservative (antifungal agent) used in food products. It is natural antimicrobial and NATURAL (produced by bacteria). NATURAL antifungal produced by soil bacterium Streptomyces natalensis. Named after Natal, South Africa where the bacteria was discovered. Also called 'pimaricin' (from Pietermaritzburg, South Africa). Works ONLY on the surface - not meant to penetrate into food. The white coating you see on some cheese rinds is often natamycin. Extremely effective against yeasts and molds but has NO effect on bacteria. Does NOT promote antibiotic resistance because it's only used on surfaces and breaks down quickly. Used in medicine as antifungal for eye infections (not taken orally).
Where is Natamycin found naturally?
Natamycin is naturally found in Produced by soil bacterium Streptomyces natalensis. NATURAL antifungal produced by soil bacterium Streptomyces natalensis. Named after Natal, South Africa where the bacteria was discovered. Also called 'pimaricin' (from Pietermaritzburg, South Africa). Works ONLY on the surface - not meant to penetrate into food. The white coating you see on some cheese rinds is often natamycin. Extremely effective against yeasts and molds but has NO effect on bacteria. Does NOT promote antibiotic resistance because it's only used on surfaces and breaks down quickly. Used in medicine as antifungal for eye infections (not taken orally).
What is the ADI for Natamycin?
The Acceptable Daily Intake (ADI) for Natamycin is 0–0.3 mg/kg bw/day as established by JECFA (Joint FAO/WHO Expert Committee on Food Additives). ADI represents the amount that can be consumed daily over a lifetime without appreciable health risk.
What foods contain Natamycin?
Natamycin is used in various food categories including Cheese (surface treatment), Dried sausages (surface treatment). It is used as a preservative (antifungal agent) in these products.